Development Of ToLCV-Resistance Transgenic Tomato Plants By amiRNAs

Development Of ToLCV-Resistance Transgenic Tomato Plants By amiRNAs

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Diseases caused by geminiviruses which are transmitted by whiteflies, leafhoppers or treehoppers greatly affect the yield of infected crops leading to enormous economic loss. The diseases have been so serious to their host crops that novel combating methods need to be developed in order to contain them. The discovery of RNAi, especially the microRNA (miR) mechanism, has opened a new era for plant virus combat since amiRs have been implicated as useful candidates to silence genes of agricultural interests. And transgenic plants harboring artificial miRs (amiRs) have shown brilliant promises of virus resistance. As the pre-coat (AV2) protein is also a candidate RNAi suppressor encoded by a geminivirus, Tomato leaf curl virus (ToLCV) and overlaps with the coat protein gene AV1, in the present study, we sought to investigate whether the AV2-amiR strategy could be extended further in a similar manner to achieve broad spectrum resistance against ToLCVs. We designed and applied amiRs for targeting the AV1 and AV2 genes of ToLCV and generated tomato transgenic lines which would over express the amiRs. These lines were then used for testing ToLCV resistance under glass-house conditions.

Alpha

Alpha

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Alpha, Bravo, Charlie… Since 1956, whenever time and clarity are of the essence, everyone from fire fighters to air traffic controllers has spelled out messages using the NATO phonetic alphabet. Now, with equal precision – infused with a singular wit and whimsy – the award-winning Isabelle Arsenault, illustrator of Jane, the Fox, and Me, interprets this internationally recognized code and makes it her own. From the elegant Tango to the enigmatic Echo, from the humorous Kilo to the haunting Romeo and Juliet, the striking art in this remarkable ABC book elicits laughter and curiosity, calls up endless associations, and will draw the reader back again and again.

Middle Eastern Patterns to Colour

Middle Eastern Patterns to Colour

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Explore the beautiful, flowing, organic designs of traditional middle eastern art and culture in this gorgeous book full of stylish patterns to colour. More than just a colouring book, this is a unique introduction to middle eastern art for older children and adults. An interactive way of finding out about the images, motifs and colours typical of art in the middle east. Part of a collectable series of art colouring books.

The China Price: The True Cost of Chinese Competitive Advantage

The China Price: The True Cost of Chinese Competitive Advantage

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In this landmark work of investigative reporting, former Financial Times correspondent Alexandra Harney uncovers a story of immense significance to us all: how China’s factory economy gains a competitive edge by selling out its workers, environment, and future. Harney’s firsthand reporting brings us face-to-face with a world in which intense pricing pressure from Western companies combines with ubiquitous corruption and a lack of transparency to exact a staggering toll in human misery and environmental damage. This eye-opening expose offers, for the first time, an intimate look at the defining business story of our time.

Окружающий мир. 3 класс

Окружающий мир. 3 класс

Автор: И. Ф. Яценко

В пособии представлены контрольно-измерительные материалы по курсу «Окружающий мир» для 3 класса в тестовой форме. Все задания соответствуют программе общеобразовательных учреждений и требованиям к уровню подготовки учащихся. Систематическая работа с материалами сборника позволит качественно подготовить учащихся к государственной аттестации.Издание адресовано учителям начальных классов, школьникам и их родителям.

Коктейль со смертью

Коктейль со смертью

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Это почти сенсация для поклонников сериала «Детективы»: теперь многие из полюбившихся историй, причем в значительно расширенном варианте, можно будет не только посмотреть, но и… прочитать. «Коктейль со смертью» — первая книга новой серии.